Document Details

Document Type : Article In Journal 
Document Title :
Solubilization and Partial Purification of the Plasma Membrane ATPase of Rhodotorula glutinis
الإذابة والتنقية الجزئية لإنزيم الأتيبيز من الغشاء البلازمي لخميرة الرودوتيلا جليتينس
 
Subject : Biochemistry 
Document Language : English 
Abstract : The optimal conditions for solubilization of the ATPase activity from the plasma membrane of Rhodotorula glutinis by zwitterionic detergent Z-3-14 were found to be: 0.55 mg/ml (5X cmc) of Z-3-14, at room temperature in the presence of 1 mM ATP, 40% (v/v) glycerol, 11.25% ammonium sulfate, 5 mM 2- mercaptoethanol, 1mM PMSF in 0.1 M Mes-Tris pH 6.5, followed by sonication in a water bath sonicator for 6 min. A 4-fold purification of the solubilized ATPase activity was obtained by centrifugation of the Z-3-14 extract through a glycerol gradient (20-50%, v/v). 
ISSN : 1012-1319 
Journal Name : Science Journal 
Volume : 17 
Issue Number : 1 
Publishing Year : 1425 AH
2005 AD 
Number Of Pages : 13 
Article Type : Article 
Added Date : Sunday, October 11, 2009 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
طه عبدالله قمصاني TAHA A. KUMOSANIResearcher  
شارلي جرفينCHARLES C. GRIFFINResearcher  

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